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Standardized perfusion protocol for swine organs based on alanine as a radiolabelled precursor.
The purpose of this study was to set up a standardized perfusion protocol for the swine organs, which includes a perfusion solution that can be replaced by another in case of organ failure. Therefore, five healthy Landrace/Large White crossbred pigs (22.4 ± 0.8 kg) were infused with 500 mL 0.9% saline followed by 5 mL of (99m)Tc-albunate (n = 5) or 5 mL (99m)Tc-albumin (n = 5) via the jugular vein, respectively. Heart, lungs, liver, spleen and kidneys were removed at 2, 5 and 10 min after the start of the infusion and tissue samples were obtained and counted for all five organs. Under the described experimental conditions, the kidney uptake was significantly lower than the values in pigs of the same weight class reported by other authors. Although the uptake in the other four organs investigated in the present study was within the reported values of the literature, only the values of lungs and liver tissue obtained with the (99m)Tc-albunate were comparable.Q:
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